Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato

Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.     

树莓果实总RNA提取方法的比较研究

比较了SDS法和Trizol法提取树莓果实总RNA的效果。结果表明:Trizol法提取的RNA经电泳检测,未见条带,说明Trizol法并不适合富含多糖多酚的树莓。SDS方法提取树莓果实总RNA完整性好,28S和18S条带清晰,OD260/OD280值为1.83,在1.7～2.0之间,OD260/OD230值为2.01。结果证明,SDS法提取的总RNA可直接用于分子克隆等分子生物学实验。     

Effect of siRNA-mediated Smad3 silence on proliferation and apoptosis in activated hepatic stellate cells

AIM: To investigate the effects of siRNA-mediated Smad3 silence on proliferation and apoptosis in activated hepatic stellate cells (HSCs).METHODS: HSCs-T6 cells were divided into 3 groups: blank group, negative control group and siRNA-Smad3 transfection group. The siRNA-Smad3 was transfected into HSCs-T6 cells. At different time points after transfection, cell proliferation was measured by CCK-8, cell apoptosis was detected by flow cytometry, and protein levels of P53 and Bcl-2 were determined by immunocytochemistry.RESULTS: HSCs proliferation was significantly inhibited at the time points of 24 h, 48 h and 72 h after transfection. Meanwhile, the apoptosis of HSCs was significantly increased in siRNA-Smad3 transfection group (P<0.01). Compared to the control cells, the protein expression of P53 was significantly increased while Bcl-2 protein was significantly decreased 48 h after transfection in siRNA-Smad3 transfection group (P<0.01).CONCLUSION: The siRNA-mediated Smad3 silence significantly inhibits HSCs proliferation and induces apoptosis by up-regulating the P53 expression and down-regulating the Bcl-2 expression in HSCs.     

散生类竹子地下鞭系生长影响因子研究综述

在搜集、分析大量相关文献的基础上,就影响散生类竹地下鞭系生长的主要因素,如林分气象、林地土壤、地形条件及林分经营干扰等因子,论述了目前我国在这一领域的研究现状,指出了研究中的薄弱环节,并提出今后的重点研究方向。     

反义PSY基因植物表达载体的构建及其对中国水仙的转化

实验从中国水仙花瓣中分离得到该基因的572bp的片段，将该片段反向连接到pCAMBIA1301双元载体质粒上，得到CaMV 35S组成型启动子的表达载体pCAM35S-PSY，用“冻融法”将pCAM35S-PSY质粒转入农杆菌LBA4404和EHA105两个菌株中。PCR扩增和酶切鉴定结果表明，所构建的反义表达载体pCAM35S-PSY是正确的并已经成功导入农杆菌中。随后，按照业已建立的遗传转化体系对中国水仙进行了遗传转化，得到了转基因抗性芽。     

一种适合杨树RNA提取的方法

本试验为三种杨树材料建立了一种杨树RNA提取的方法。本方法具有充分抑制材料中的多酚褐化,提高RNA产率;最大限度地去除多糖及次生代谢物质,避免与RNA共沉淀;去除蛋白质和DNA污染;提取时间短,质量高等特点。通过本方法提取的RNA OD260/OD280值在1.9~2.0之间,能够完全满足后续实验的要求,如体外翻译、cDNA文库构建、基因芯片和DDRT-PCR等。     

改进邻体干扰模型在北京侧柏水源涵养林中的应用

以北京市密云半城子侧柏水源涵养林为研究对象,提出了改进的邻体干扰模型,着重讨论了干扰指数与侧柏的胸径、树高、生境利用率等因子的关系。结果表明,侧柏林内的邻体干扰指数与林木的胸径、树高、生境利用率呈负相关关系。本研究为该地区水源涵养林的经营提供了具有参考价值的量化依据。     

涌泉根灌多点源交汇入渗湿润体试验研究

在米脂山地微灌枣树示范基地进行原状土涌泉根灌入渗试验,研究了多点源交汇入渗条件下涌泉根灌湿润体特征值的变化规律.结果表明,涌泉根灌多点源交汇入渗孔洞处和交汇面处的湿润锋运移距离与入渗时间均符合幂函数关系,交汇面处的湿润锋运移速度比孔洞处的快,最终交汇入渗湿润土体沿孔洞布置方向的剖面形状近似带状;在孔洞底部周围的中间区域...     

考虑部件弹性变形的平衡悬架运动干涉分析

研究了某特种重型车三轴平衡悬架在考虑车架、摆臂等主要部件弹性变形基础上的运动干涉校核问题,建立了基于整车的有限元计算模型并进行了计算分析。计算分析结果表明,对于特殊情况下不考虑车架等主要部件的弹性变形进行多轴平衡悬架运动干涉校核是不充分的,可能会得到不正确的结果。     

变压器局部放电在线监测技术分析

阐述了变压器局部放电在线监测的原理、现状以及在线监测方案如何选择，分析了变压器局部放电在线监测中的各种干扰及其抑制方法并提出了实际监测中分层式抑制干扰的模式，探析了目前在线监测局部放电所存在的问题。